Isabelle Viana

Isabelle Viana Post Doctoral Associate
Center for Vaccine Research (CVR)
9052 BST3
3501 Fifth Avenue
Pittsburgh, Pennsylvania 15261


Phone: (412) 624-3804
Fax: (412) 624-4440
E-mail: viana@pitt.edu
Webpage: http://www.cvr.pitt.edu/personnel/view.asp?uid=viana
Biography

      Isabelle Viana obtained a Bachelor's Degree in Biomedicine in 2009 at the Federal University of Pernambuco in Brazil. In 2011, Dr. Viana obtained a Master of Science Degree in Genetics and Molecular Biology at the same university in Brazil. In 2014, Dr. Viana obtained a Ph.D. degree through the Osvaldo Cruz Foundation in Brazil and the University of Pittsburgh in USA. In the same year, Dr. Viana became a Visiting Researcher at the Center for Vaccine Research at the University of Pittsburgh. In 2015, Dr. Viana was appointed as a Postdoctoral Associate at the Infectious Diseases and Microbiology Department at the University of Pittsburgh. As an early career scientist, Dr. Viana has been building her portfolio on the design and characterization of proteins to address biomedical applications. She has applied computational chemistry tools to design and simulate proteins and peptides with specific biological functions, and applied molecular biology, structural bioinformatics and biophysical tools to determine the structural dynamics and biophysical parameters of those proteins. She has expertise in experimental methods in molecular biology, notably in protein production and high throughput immune assays, and knowledge in experimental methods in molecular biophysics, such as nuclear magnetic resonance, circular dichroism and surface plasmon resonance. She has also good experience with immunology, particularly in humoral response profiling, function, isolation and characterization of B-cells, in vitro production of antibodies and immunoassays development. Her current interests are the engineering of novel vaccine molecules, the development of diagnostic markers of viral infections and the understanding of immunological parameters governing epitope presentation, antigen-antibody interactions and humoral response. She has been applying her skills to address medical questions related to infectious diseases of global importance, such as HIV, HTLV, Dengue, Yellow Fever and Zika.

Selected Publications

  • Viana, Isabelle; Nascimento, Eduardo; Craigo, Jodi; Krieger, Marco; Dhalia, Rafael; Lins, Roberto; Maillard, Robbie; Rinaldo, Charles; Marques, Ernesto. De Novo Design and Biophysical Characterization of an Affinity-Enhanced Protein Displaying the Structure of the Broadly Neutralizing HIV-1 2F5 Antibody Epitope. Biophysical Journal. 2016 Jan. DOI: 10.1016/j.bpj.2015.11.1860


  • Gu, G; Coelho, D; Lins, R; Khan, A; Viana, IFT; Cordeiro, M; Chiang, J; Carvalho, V; Braga-Neto, U; Marques, E; Nascimento, E. Protection Against Disease Severity Induced by IgG targeting a B cell Epitope on Dengue NS1 Protein. Journal of Virology, submitted on 01/2016.


  • Nascimento, E; Viana, IFT; Cassemiro, K; Marques, E. Development of Mono and Multiplex Immune-assays for Quantification of Complement Proteins. PLOS ONE, submitted on 12/2015.


  • Cunha KC, Rusu VH, Viana IF, Marques ET, Dhalia R, Lins RD. Assessing protein conformational sampling and structural stability via de novo design and molecular dynamics simulations. Biopolymers. 2015 Jun;103(6):351-61. PubMed PMID: 25677872.


  • Viana, Isabelle FT; Dhalia, Rafael; Krieger, Marco; Marques JR., Ernesto TA; Lins, Roberto. Influence of Scaffold Stability and Electrostatics on Top7-Based Engineered Helical HIV-1 Epitopes. In: Advances in Bioinformatics and Computational Biology Volume 8213 LNBI, 2013. 8th Brazilian Symposium on Bioinformatics, BSB 2013; Code 100552; 2013 Nov 3-7; Recife; Brazil;. Springer International Publishing, 2013 Nov 1. 94-103. doi: 10.1007/978-3-319-02624-4_9.


  • Viana, Isabelle FT ; Soares, Thereza A. ; Lima, Luciana FO ; Marques JR., Ernesto TA ; Krieger, Marco ; Dhalia, Rafael ; Lins, Roberto . De Novo Design of Immunoreactive Conformation-specific HIV-1 Epitopes based on Top7 Scaffold. RSC Advances: an international journal to further the chemical sciences, v.3, p.11790 - 11800, 2013.


  • G. Bezerra-Jr., A. Barison, V. S. Oliveira, L. Foti, M. A. Krieger, R. Dhalia, I. F. T. Viana and W. H. Schreiner. The mechanism of cysteine detection in biological media by means of vanadium oxide nanoparticles. Journal of Nanoparticle Research, v.14, p.1123 - 1133, 2012.


  • Dhalia, R.; Maciel Jr., M.; Cruz, F.S.P.; VIANA, I.F.T.; Palma, M.L.; August, T.; Marques Jr., E.T.A. Membrane and envelope virus proteins co-expressed as lysosome associated membrane protein (LAMP) fused antigens: a potential tool to develop DNA vaccines against flaviviruses. Anais da Academia Brasileira de Ciências (Impresso). , v.81, p.663 - 669, 2009.





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