Eduardo Nascimento, PhD

Eduardo Nascimento Post-Doctoral Associate
Center for Vaccine Research (CVR)
9052 BST3
3501 Fifth Avenue
Pittsburgh, Pennsylvania 15261

  Curriculum Vitae
  Google Scholar

Phone: (412) 624-3804
Fax: (412) 624-4440

      I received my Bachelor's degree on Medical Technology at Federal University of Pernambuco (UFPE), Recife, Brazil in December 1999. Then in October 2003 I earned my PhD in Molecular and Cellular Biology from Oswaldo Cruz Institute (IOC/FIOCRUZ) also in Brazil, studying the immunogenicity of different DNA vaccines against Schistosoma mansoni in a murine model. During my post-doctoral experience I had a chance to work in a multidisciplinary group aimed to study different aspects involved on dengue immunopathology. Among the studies I was involved included the analysis of transcriptome of immune cells collected during acute phase of dengue infection from subjects with different clinical outcome as well as the identification of immunoprevalent T cell epitopes in people with history of dengue infection and Yellow fever vaccinees. In addition, I also worked on a project aimed to map dengue T cell epitopes, using ELIspot for IFNg as a read out for T cell activation, in HLA transgenic mice using overlapping peptides covering almost entirely the dengue virus proteome. I also have experience on flow cytometry analysis using FACS Calibur and LSRII. Currently my research focus has been on development and validation of immunoassays, such as ELISA and liquid microarrays (luminex), to allow the assessment of the status of complement activation on human sera/plasma as well as on culture supernatant. So far, ELISA assays to quantify more than 20 different complement proteins have been optimized and half of that is also available for liquid microarray using an alternative approach we developed for coupling antibodies to seroMAP beads. These tools will be important to investigate not only the role of complement activation on dengue pathogenesis (which is my primary interest) but also any condition where this arm of innate immune system is involved.

Selected Publications

  • Ole Lund, Eduardo J. M. Nascimento, Milton Maciel, Jr, Morten Nielsen, Mette Voldby Larsen, Claus Lundegaard, Mikkel Harndahl, Kasper Lamberth, Søren Buus, Jérôme Salmon, Thomas J. August, and Ernesto T. A. Marques, Jr. Human Leukocyte Antigen (HLA) Class I Restricted Epitope Discovery in Yellow Fewer and Dengue Viruses: Importance of HLA Binding Strength. PLoS One, v.6(11); 2011.

  • Nascimento, E.J.M.; Braga-Neto, U ; Calzavara, C.; Gomes, A. L.; Abath, F; Acioli, B; Brito, C.A.A.; Cordeiro, M.; Silva, A.; Magalhaes, C; Andrade, R; Gil, L.; Marques Jr, E.T.A. Gene expression profiling during acute stage of dengue infection can predict patient outcome. PLoS One, vol. 4 (11) pp. e7892, 2009.

  • Eduardo J. M. Nascimento, Ana M. Silva, Marli T. Cordeiro, Carlos A. A. Brito, Laura H. V. G. Gil, Ulisses Braga-Neto and Ernesto T. A. Marques Jr. Alternative Complement Pathway Deregulation Is Correlated with Dengue Severity. PLoS One 4:e6782, 2009.

  • Andréa N. M. Rangel da Silva, Eduardo J. M. Nascimento, Marli Tenório Cordeiro, Frederico G.C. Abath, Silvia M.L. Montenegro, Ernesto T. A. Marques Junior. Identification of Continuous B-cell Epitopes in the Glycoprotein Envelope of Dengue Virus Type 3 (DENV-3). PLoS One, vol. 4 (10) pp. e7425, 2009.

  • Asif M. Khan, Olivo Miotto, Eduardo J. M. Nascimento, J. Salmon, K.N. Srinivasan, A.T. Heiny, Guang Lan Zhang, E.T. Marques, Tin Wee Tan, Vladimir Brusic, J. Thomas August. Conservation and Variability of Dengue Virus Proteins: Implications for Vaccine Design PLoS Negl Trop Dis 2(8): e272, 2008.

  • Marli Tenório Cordeiro, Ana Maria Silva, Carlos A A Brito, Eduardo J M Nascimento, Maria Cecilia F Magalhães, Georgia F Guimarães, Norma Lucena-Silva, Eduardo Freese de Carvalho, Ernesto T A Marques, Jr. Characterization Of A Dengue Patient Cohort In Recife, Brazil. Am J Trop Med Hyg, 77(6): 1128-1134, 2007.

  • Asif M. Khan, Olivo Miotto, A.T. Heiny, Jerome Salmon, K.N. Srinivasan, Eduardo J M Nascimento, Ernesto T. Marques, Vladimir Brusic, Tin Wee Tan, J. Thomas August. A systematic bioinformatics approach for selection of epitope-based vaccine targets. Cellular Immunology, 244: 141-7, 2006.

  • Nascimento E.J.M, Amorim RV, Cavalcanti A, Alves VF, Nakazawa M, Pereira VR, Lucena-Silva N. Assessment of a DNA vaccine encoding an anchored-glycosylphosphatidylinositol tegumental antigen complexed to protamine sulphate on immunoprotection against murine schistosomiasis. Mem Inst Oswaldo Cruz,102(1):21-7, 2007.

  • Nascimento E, Pereira VRA, Gomes YM, Chikhlicar P, August A, Marques E, Lucena-Silva N. Protective immunity of single and multi-antigen DNA vaccines against schistosomiasis. Memórias do Instituto Oswaldo Cruz, 97 (suppl. I): 105-109, 2002

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