Jodi Kristine Craigo, PhD
Research Manager, CVR|
Research Associate Professor
Microbiology & Molecular Genetics
3501 Fifth Avenue
Pittsburgh, Pennsylvania 15261
Phone: (412) 624-4610
Fax: (412) 624-4440
Dr. Craigo is a Research Associate Professor. In 2007 she took the position of Research Manager of the CVR. She oversees the daily operations of the CVR which includes management of the core staff, equipment, and CVR facilities in the BST3. She is responsible for the design and daily content of the CVR social media marketing. She has been faculty in the Department of Microbiology & Molecular Genetics at the University of Pittsburgh School of Medicine since 2006. Jodi received her Ph.D. in Cellular and Molecular Biology in 1997 from Northeastern Ohio Universities College of Medicine & Kent State University in Rootstown, Ohio. She came to Pittsburgh in January of 1998 as a Post-Doctoral Research Associate in the laboratory of Dr. Ronald Montelaro. Her research interests include antimicrobial peptide development as well as utilizing SPR to answer mechanistic questions especially towards microbe pathogenesis, antibody:antigen interactions and vaccine development. Dr. Craigo additionally directs the biosensor/SPR core at the CVR that currently involves several collaborations with diverse departments and topics in the area of biomolecular interaction analysis.
My research started in the characterization of lentiviral persistence and pathogenesis, conducted through evolutionary studies of both HIV and EIAV sequence analysis. Our evolutionary analysis of lentiviral populations via quasispecies sequence analysis included determinations of phenotypic differences of conserved and immunodominant regions of protein variation utilizing multiple computational avenues including: phylogenetics, divergence and selection analyses, and compartmentalization analyses (including Slatkin-Maddison, Mantel and GeneFlow computations). I still serve as the Project Manager for the Montelaro lab for all vaccine development grants (NIH). Our lentiviral vaccine development includes various modalities such as live attenuated, viral vector and virus like particle vaccines. Our vaccine research applied what we learn from pathogenesis studies into mechanisms of protection and results ultimately in the implementation of the new candidate vaccines into trials. In addition to the development of vaccine candidates, we also focus on the creation of new, rigorous virus challenge models. A major focus of my independent research has recently been focused in the detailed characterization of dynamic antigen-antibody interactions that included analyzing protective/non-protective immune system responses; specifically utilizing SPR analysis for the development of novel immune assays and characterizations of the structure:function relationship to correlates of protection. Most recently I've started working on the development of antimicrobial peptides against drug-resistant bacteria, also utilizing SPR to dissect peptide membrane interactions and evaluate mechanisms of efficacy.
- Craigo, J.K., Zhang, B., Barnes, S., Tagmyer, T., Cook, S., Issel, C., and Montelaro, R.C. (2007) Envelope Variation as a Primary Determinant of Lentiviral Vaccine Efficacy. Proc. Nat. Acad. Sci. 104:15105-15110. | Abstract
- Craigo JK, Ezzelarab C, Cook SJ, Chong L, Horohov D, Issel CJ, Montelaro RC. (2013) Envelope determinants of equine lentiviral vaccine protection.PLoS One.8(6):e66093. doi: 10.1371/journal.pone.0066093. Print 2013.
- Craigo JK, Steckbeck JD, Barnes CO, Montelaro RC. (2011) Highly conserved structural properties of the C-terminal tail of HIV-1 gp41 protein despite substantial sequence variation among diverse clades: implications for functions in viral replication. J Biol Chem. 286:27156-27166. | Abstract
- Craigo, J.K., Barnes, S., Zhang, B., Cook, S.J., Howe, L., Issel, C., and Montelaro, R.C. (2009) An EIAV Field Isolate Reveals Much Higher Levels of Subtype Variability Than Currently Reported for the Equine Lentivirus Family. Retrovirology 6: 95. | Abstract
- Craigo, J.K., Durkin, S., Sturgeon, T., Tagmyer, T., Cook, S., Issel, C., and Montelaro, R.C. (2007) Immune suppression of challenged vaccinates as a rigorous assessment of sterile protection by lentiviral vaccines. Vaccine. 25:834-835. | Abstract
- Craigo, J.K. and Gupta, P. (2006) HIV-1 in Genital Compartments: Vexing Viral Reservoirs. Current Opinion in HIV and AIDS. 1:97-102. | Abstract
- Craigo, J.K., Li, F., Howe, L., Steckbeck, J.D., Cook, S.J., Issel, C., and Montelaro, R.C. (2005) Discerning An Effective Balance Between Equine Infectious Anemia Virus Attenuation And Vaccine Efficacy. Journal of Virology. 79: 2666-2677. | Abstract
- Craigo, J.K., Patterson, B., Paranipe, S.G., Ding, M., Kulka, K., Montelaro, R.C., Mellors, J., and Gupta,P. (2004) Persistent HIV type 1 infection in semen and blood compartments in patients after long-term potent antiretroviral therapy.. AIDS Research and Human Retroviruses. 20:1196-1209. | Abstract
- Paranipe, S.G., Craigo, J., Patterson, B., Ding, M.,Barroso, P., Harrison, L., Montelaro, R.C., and Gupta, P. (2002) Subcompartmentalization of HIV-1quasispecies between seminal cells and seminal plasma indicates their origin in distinct genital tissues. AIDS Research and Human Retroviruses. 18: 1271-1280. | Abstract
- Craigo, J. K., Leroux, C., Howe, L., Steckbeck, J.D., Cook, S.J., Issel, C. J., and Montelaro, R.C. (2002) Transient immune suppression of inapparent carriers infected with a principal neutralizing domain-deficient equine infectious anemia virus induces neutralizing antibodies and lowers steady-state replication. Journal of General Virology. 83:1353-1359. | Abstract